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Gq protein
signaling
cascade
Phospholipase C
Inositol 1,4,5-
trisphosphate
receptor type 1
Protein kinase
C alpha type
Voltage-
dependent
P/Q-type
calcium channel
subunit
alpha-1A
Voltage-
dependent
L-type calcium
channel subunit
beta-1
Voltage-
dependent
calcium channel
subunit
alpha-2/delta-2
Myosin light
chain kinase,
smooth muscle
Muscarinic
acetylcholine
receptor M3
Myosin LC-P
Myosin light
chain 3
Calmodulin-1
Serine/threonine-
protein
phosphatase
PP1-beta
catalytic
subunit
Calmodulin-1
Inositol 1,4,5-trisphosphate
Pilocarpine
Ca
+
Ca
+
Ca
+
Phosphatidylinositol
4,5-bisphosphate
Calcium
Ca
+
Diacylglycerol
Muscle
Contraction
Muscle
Relaxation
Magnesium
Calcium
Manganese
Sarcoplasmic Reticulum
Myosin binds to actin
causing muscle contraction
Increased calcium binds
readily to calmodulin to
activate myosin light chain
kinase
Activation of myosin light
chain kinase leads to high
concentration of myosin LC-P
which causes contractions of
the muscles
There is an overall increase
in calcium levels in the
cytosol caused by the
activation of the Gq
signaling cascade
Cytosol
Ciliary muscle or Iris
sphincter muscle
Pilcarpine is an agonist of
the Muscarinic acetylcholine
receptor M3
GNAQ
PLCB1
ITPR1
PRKCA
CACNA1A
CACNB1
CACNA2D2
MYLK
CHRM3
MYL3
MYL3
CALM1
PPP1CB
CALM1
Inositol 1,4,5-
trisphosphate
Pilocarpine
Calcium